Beta Amyloid 1-16 Mouse Polyclonal Antibody

SKU: orb500591

Description

Beta Amyloid 1-16 Mouse Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human
• Predicted Reactivity: Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide of human beta-Amyloid (1-16/42aa)
• Target: APP
• Molecular Weight: 4.3/83 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AAA; ABETA; ABPP; AD1; APPI; CTFgamma; CVAP; PN-II; PN2; alpha-sAPP; preA4; Adap; Ag; E030013M08Rik; betaApp; A4_HUMAN; APP; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide (CVAP); Protease nexin-II (PN-II); A4; A4_RAT; ABPP (APP); Amyloidogenic glycoprotein (AG);

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (beta Amyloid 1-16) Monoclonal Antibody, Unconjugated (orb500591) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human U87MG Cell Lysates, Lane 2: Human SY5Y cell lysates, Lane 3: Human U251 Cell Lysates, Primary: Anti-beta Amyloid 1-16 (orb500591) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 4.3/83 kDa, Observed band size: 130 kDa.

Quick Database Links

Gene Symbol

APP

UniProt

P05067