ALPL antibody

• Catalog Number: orb1436162
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ALPL.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB14192
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 57305
• Target: ALPL
• UniProt ID: P05186
• NCBI: NP_000469.3, NP_001120973.2, NP_001170991.1
• Alternative names: Alkaline phosphatase, tissue-nonspecific isozyme,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescent analysis of ALPL Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Western blot:All lanes:Anti-ALPL Antibody (Center) at 1:2000 dilution.Lane 1:Hela;Lane 2:F9.

Western blot:Anti-ALPL Antibody (Center) at 1:2000 dilution + F9.Secondary antibody was Goat Anti-Rabbit IgG, (H+L).

Immunohistochemical analysis of human liver tissue using orb1436162 performed on the Leica® BOND RXm at 1:500 dilution.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of human kidney tissue using orb1436162 performed on the Leica® BOND RXm at 1:500 dilution.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Flow cytometric analysis of 293 cells (right histogram) using ALPL Antibody (Center), compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.