ALPL Antibody

• Catalog Number: orb1252202
• Category: Antibodies
• Description: ALPL Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ALPL/597
• Tested applications: FC, IF, IHC-P
• Reactivity: Bovine, Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human ALPL protein was used as the immunogen for the Alkaline Phosphatase antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted format : incubate for 30 min at RT (1)Optimal dilution of the Alkaline Phosphatase antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ALPL
• UniProt ID: P05186
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: ALPL, Alkaline phosphomonoesterase, AP-TNAP, Alkal
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted format : incubate for 30 min at RT (1)Optimal dilution of the Alkaline Phosphatase antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human colon carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human endometrial carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human testicular carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human ovarian carcinoma with Alkaline Phosphatase antibody (ALPL/597). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.