Alpha Catenin Antibody

• Catalog Number: orb385946
• Category: Antibodies
• Description: Recognizes a protein of 102kDa, identified as Catenin, alpha-1. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-Catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, alpha-Catenin forms a heterodimer with beta-Catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-Catenin also regulates the beta-Catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1G5
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: A human fusion protein was used as the immunogen for the Alpha Catenin antibody.
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Alpha Catenin antibody is available for research use only.
• UniProt ID: P35221
• Storage: Store the Alpha Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Alpha Catenin antibody to be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human A431(lane 1) and MCF7(lane 2) cell lysate using Alpha Catenin antibody