AKT antibody (Biotin)

• Catalog Number: orb344777
• Category: Antibodies
• Description: AKT antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-AKT Pan Reactive Antibody was produced in rabbits by repeated immunizations with a cocktail containing recombinant human AKT1, AKT2, and AKT3 protein isoforms.
• Concentration: 1 mg/mL
• Dilution range: ELISA: User Optimized, FC: User Optimized, IHC: 1:500 - 1:2,000, IF: User Optimized, IP: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: AKT Antibody reacts with the AKT from human tissues. Anti-AKT antibody was purified from monospecific antiserum by Protein A chromatography. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
• Conjugation: Biotin
• UniProt ID: P31749
• NCBI: NP_001014431.1
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-AKT Antibody Biotin Conjugated, Biotin
• Note: For research use only
• Application notes: Biotin Conjugated AKT Antibody is tested for western blotting to detect a single band of the expected apparent molecular weight. This antibody is suitable for immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecipitation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.