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Catalog Number | orb1274462 |
---|---|
Category | Antibodies |
Description | AIFM1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 334-613 of human AIF (NP_004199.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 67kDa |
Target | AIFM1 |
UniProt ID | O95831 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | AIF Antibody: CNK, KSR, CNK1, Connector enhancer o Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and AIF knockout (KO) 293T cells, using AIF antibody (orb1274462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat lung using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using AIF antibody (orb1274462) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using AIF antibody (orb1274462). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using AIF antibody (orb1274462) at dilution of 1:200 (60x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using AIF antibody (orb1274462) at dilution of 1:200. Blue: DAPI for nuclear staining.
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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