Cart summary

You have no items in your shopping cart.

AIF1/IBA1 isoform 1 and 3

Catalog Number: orb18542

DispatchUsually dispatched within 3-5 working days
$ 500.00
Catalog Numberorb18542
CategoryAntibodies
DescriptionGoat polyclonal antibody to Interferon gamma
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityHuman, Mouse, Porcine, Rat
Dilution rangeELISA: 1:128000, WB: 0.5-1μg/ml, IHC-P: 5μg/ml
ConjugationUnconjugated
MW10.5; 16.7
TargetAIF1/IBA1 isoform 1 and 3
Entrez199
Protein SequenceTGPPAKKAISELP
RRIDAB_10750970
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesAIF1; IBLY409840A1; allograft inflammatory factor
Read more...
NoteFor research use only
Application notesELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Mouse Brain shows distinct staining of astrocytes. Recommended concentration, 2-4µg/ml. Data obtained from a previous batch (different goat).WB: Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.1-0.3µg/ml.
Expiration Date12 months from date of receipt.

Filter by Applications

Filter by Reactivity

Applications

IF

Reactivity

Rat
AIF1/IBA1 isoform 1 and 3

1 µg/ml staining of Mouse (A) and (3 µg/ml) Rat (B) Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

AIF1/IBA1 isoform 1 and 3

6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

AIF1/IBA1 isoform 1 and 3

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

AIF1/IBA1 isoform 1 and 3

Negative Control showing staining of paraffin embedded Human Frontal Cortex, with no primary antibody.

AIF1/IBA1 isoform 1 and 3

4 µg/ml staining of paraffin embedded Human Frontal Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.