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Catalog Number | orb18542 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to Interferon gamma |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 0.5-1μg/ml, IHC-P: 5μg/ml |
Conjugation | Unconjugated |
MW | 10.5; 16.7 |
Target | AIF1/IBA1 isoform 1 and 3 |
Entrez | 199 |
Protein Sequence | TGPPAKKAISELP |
RRID | AB_10750970 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | AIF1; IBLY409840A1; allograft inflammatory factor Read more... |
Note | For research use only |
Application notes | ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Mouse Brain shows distinct staining of astrocytes. Recommended concentration, 2-4µg/ml. Data obtained from a previous batch (different goat).WB: Approx 17kDa band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.1-0.3µg/ml. |
Expiration Date | 12 months from date of receipt. |
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Song-I Seol 1, Sang-A Oh 1, Dashdulam Davaanyam 1, Ja-Kyeong Lee Blocking peptidyl arginine deiminase 4 confers neuroprotective effect in the post-ischemic brain through both NETosis-dependent and -independent mechanisms Acta Neuropathol Commun, (2025)
Applications
Reactivity
1 µg/ml staining of Mouse (A) and (3 µg/ml) Rat (B) Brain lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.
6 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
Negative Control showing staining of paraffin embedded Human Frontal Cortex, with no primary antibody.
4 µg/ml staining of paraffin embedded Human Frontal Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
ELISA, IHC, WB | |
Human | |
Goat | |
Unconjugated |
ELISA, WB | |
Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Rat | |
Goat | |
Unconjugated |
ELISA, WB | |
Mouse | |
Goat | |
Unconjugated |