Adiponectin antibody

• Catalog Number: orb10048
• Category: Tools
• Description: Adiponectin antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Adiponectin (151-244/244 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: Adiponectin
• UniProt ID: Q60994
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: 30 kDa adipocyte complement related protein; 30 kD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Du, Jingjing et al. MicroRNA-125a-5p Affects Adipocytes Proliferation, Differentiation and Fatty Acid Composition of Porcine Intramuscular Fat Int J Mol Sci, 19, E501 (2018)

PubMed: 29414921

Western blot analysis fo rat liver lysates(Lane1 30ug) and mouse intestine lysates(Lane2 30ug) using Adiponectin antibody

Paraffin-embedded mouse liver tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). Adiponectin antibody at 1:200 dilution with overnight incubation at 4 degrees