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Catalog Number | orb137986 |
---|---|
Category | Antibodies |
Description | Adiponectin/ADIPOQ Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Predicted Reactivity | Hamster |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ADIPOQ (224-244aa LYADNDNDSTFTGFLLYHDTN), different from the related mouse and rat sequences by one amino acid. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | ELISA , 0.1-0.5μg/ml, Human, -Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, MouseImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Rat, Mouse |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 26414 MW |
UniProt ID | Q15848 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Adiponectin;30 kDa adipocyte complement-related pr Read more... |
Note | For research use only |
Application notes | WB: The detection limit for ADIPOQ is approximately 1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Kidney Tissue using ADIPOQ antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Mouse Kidney Tissue using ADIPOQ antibody
Western blot analysis of Lane 1: Recombinant Human ADIPOQ Protein 10ng, Lane 2: Recombinant Human ADIPOQ Protein 5ng, Lane 3: Recombinant Human ADIPOQ Protein 2.5ng using ADIPOQ antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Human Placenta Tissue using ADIPOQ antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Liver Tissue using ADIPOQ antibody
Western blot analysis of Rat Heart Tissue Lysate using ADIPOQ antibody
IHC-P, WB | |
Bovine, Canine, Gallus, Porcine, Rabbit |
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