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Catalog Number | orb1239163 |
---|---|
Category | Antibodies |
Description | ADIPOQ Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Predicted Reactivity | Bovine |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Adiponectin antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human adiponectin.The immunogen is located within the last 50 amino acids of Adiponectin. |
Concentration | 1 mg/mL |
Dilution range | Adiponectin antibody can be used for the detection of adiponectin by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 27 kDa Observed: 26 kDa |
Target | ADIPOQ |
UniProt ID | Q15848 |
NCBI | NP_004788 |
Storage | Adiponectin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Adiponectin Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | Adiponectin Antibody: ACDC, ADPN, APM1, APM-1, GBP Read more... |
Note | For research use only |
Application notes | Adiponectin antibody can be used for the detection of adiponectin by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of adiponectin in HL60 cell lysate with adiponectin antibody at (A) 0.5, (B) 1, and (C) 2 µg/mL.
Immunohistochemistry of adiponectin in rat brain tissue with adiponectin antibody at 10 µg/mL.
Immunofluorescence of Adiponectin in Rat Brain cells with Adiponectin antibody at 10 µg/mL.
Immunohistochemistry of Adiponectin in mouse brain tissue with Adiponectin antibody at 5 µg/ml.
Immunofluorescence of Adiponectin in mouse brain tissue with Adiponectin antibody at 20 µg/mL. Red: Adiponectin Antibody (orb1239163) Blue: DAPI staining.
ELISA, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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