Adenosine Deaminase antibody

• Catalog Number: orb344598
• Category: Antibodies
• Description: Adenosine Deaminase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IP, WB
• Reactivity: Bovine
• Isotype: IgG
• Immunogen: Adenosine Deaminase [Calf Spleen]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Adenosine Deaminase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P56658
• NCBI: NP_776312.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Adenosine Deaminase Antibody, Adenosin
• Note: For research use only
• Application notes: Anti-Adenosine Deaminase antibody has been tested in western blot and is suitable for ELISA and Immunoprecipitation applications. Anti-ADENOSINE DEAMINASE antibody was tested against 1.0 ug of Adenosine Deaminase [Calf Spleen] by immunoblot using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and DAB as a substrate at room temperature. A working dilution of 1:8,000 to 1:40,000 is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions using Adenosine Deaminase antibody