ADA Antibody (monoclonal, 6D4)

• Catalog Number: orb507552
• Category: Antibodies
• Description: ADA Antibody (monoclonal, 6D4)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6D4
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 45 kDa
• UniProt ID: P00813
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Adenosine deaminase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-ADA antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ADA using anti-ADA antibody.Lane 1:human HeLa cell;2:human placenta tissue;3:human A549 cell;4:human MCF-7 cell;5:human U-937 cell;6:human U20S cell;7:human Caco-2 cell.

IHC analysis of ADA using anti-ADA antibody.ADA was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of ADA using anti-ADA antibody.ADA was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of ADA using anti-ADA antibody.ADA was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of ADA using anti-ADA antibody.ADA was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of ADA using anti-ADA antibody.ADA was detected in paraffin-embedded section of human tonsil tissue.