ACTN4 Antibody

• Catalog Number: orb1241756
• Category: Antibodies
• Description: ACTN4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 4.
• Concentration: batch dependent
• Dilution range: Actin alpha 4 antibody can be used for detection of Actin alpha 4 by ELISA at 1:312500. Actin alpha 4 antibody can be used for detection of Actin alpha 4 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 100 kDa
• Target: ACTN4
• UniProt ID: O43707
• NCBI: NP_004915
• Storage: For short periods of storage (days) store at 4°C. For longer periods of storage, store Actin alpha 4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: ACTN4, DKFZp686K23158, FSGS, FSGS1, ACTININ-4
• Note: For research use only
• Application notes: Actin alpha 4 antibody can be used for detection of Actin alpha 4 by ELISA at 1:312500. Actin alpha 4 antibody can be used for detection of Actin alpha 4 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on ACTNX-GFP transfected at 1:1000 (Lane 1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane 2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane 3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane 4: 10 ug ACTN4-GFP transfected COS-7 lysate).

Antibody used in IHC on ACTNX-GFP transfected at 1:1000.

Antibody used in WB on Human HCT116 at 0.625 ug/ml.