Alpha Actinin 4/ACTN4 Antibody

• Catalog Number: orb371708
• Category: Antibodies
• Description: Alpha Actinin 4/ACTN4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Alpha Actinin 4 recombinant protein (Position: E561-V661). Human Alpha Actinin 4 shares 99% and 98% amino acid (aa) sequence identity with mouse and rat Alpha Actinin 4, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 104854 MW
• UniProt ID: O43707
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Alpha-actinin-4 ;Non-muscle alpha-actinin 4 ;ACTN4
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-Alpha Actinin 4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of HEPA1-6 cells using anti-Alpha Actinin 4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of C6 cells using anti-Alpha Actinin 4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody.Lane 1:human MCF-7 cell; 2:human HeLa cell; 3:human HepG2 cell; 4:huamn HT1080 cell; 5:human placenta tissue; 6:human A549 cell; 7:human U251 cell; 8:human RT-4 cell.

WB analysis using anti-Alpha Actinin 4 antibody.Lane 1:rat skeletal muscle tissue; 2:rai liver tissue; 3:rat lung tissue; 4:rat RH35 cell; 5:mouse sketetal muscle tissue; 6:mouse liver tissue; 7:mouse lung tissue; 8:mouse NIH/3T3 cell.

IF analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody. Alpha Actinin 4 was detected in an immunocytochemical section of HeLa cells.

IHC analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody. Alpha Actinin 4 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue.

IHC analysis of Alpha Actinin 4 using anti-Alpha Actinin 4 antibody. Alpha Actinin 4 was detected in a paraffin-embedded section of human prostate cancer tissue.