ACO2 antibody

• Catalog Number: orb20461
• Category: Antibodies
• Description: Goat polyclonal antibody to ACO2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:16000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml
• Conjugation: Unconjugated
• MW: 85.4
• Target: Aconitase 2 (aa541-555)
• Entrez: 50
• Protein Sequence: QDTYQHPPKDSSGQH
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti aconitase 2, mitochondrial antibody, anti ACO
• Note: For research use only
• Application notes: IHC: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5μg/ml.WB: Approx 90kDa band observed in Human Heart lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of heart lysate (35µg protein in RIPA buffer) using Aconitase 2 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human breast tissue using Aconitase 2 antibody

Western blot analysis of staining of Human (A), Mouse (B) and Rat (C) Adipose lysates using ACO2 antibody.

Western blot analysis of staining of Mouse (A), Rat (B) and Pig (C) Skeletal Muscle lysates using ACO2 antibody.