6X His Tag antibody (ATTO 647N)

• Catalog Number: orb344563
• Category: Antibodies
• Description: 6X His Tag antibody (ATTO 647N)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 33D10.D2.G8
• Tested applications: DOT, FLISA, IF, WB
• Isotype: IgG1
• Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
• Concentration: 1.0 mg/mL
• Dilution range: FLISA: >1:20,000, IF: >1:5,000, WB: >1:10,000
• Form/Appearance: Lyophilized
• Purity: 6X HIS Epitope Tag ATTO 647N conjugated antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
• Conjugation: ATTO-647N
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-6X His Tag ATTO 647N conjugated Antibod
• Note: For research use only
• Application notes: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA, dot blot, and western blotting against both the immunizing peptide and His-containing recombinant proteins. ATTOs are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using 6X His Tag antibody (ATTO 647N)

Western blot analysis of 100ng Purified histidine-tagged recombinant protein (Lane1), 200ng E. coli cell lysate containing histidine-tagged expression construct (Lane2), 100ng Purified GST-tagged recombinant protein (Lane3), 100ng Purified FLAG-tagged rec

Western blot analysis of Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged using 6X His Tag antibody (ATTO 647N)