53BP1 Antibody

• Catalog Number: orb1257346
• Category: Antibodies
• Description: 53BP1 Antibody
• Target: TP53BP1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human 53BP1 (NP_001135452.1).
• UniProt ID: Q12888
• MW: Observed: 255kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TP53BP1, 53BP1, p202, p53-binding protein 1, p53-b
• Research Area: Cancer Biology
• Note: For research use only

Western blot analysis of extracts of A-431 cells, using 53BP1 antibody (orb1257346) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 40s.

Immunohistochemistry of paraffin-embedded rat kidney using 53BP1 antibody (orb1257346) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using 53BP1 antibody (orb1257346) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse heart using 53BP1 antibody (orb1257346) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using 53BP1 antibody (orb1257346). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using 53BP1 antibody (orb1257346). Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.