ZSWIM4 Rabbit Polyclonal Antibody

SKU: orb186509

Description

ZSWIM4 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ZSWIM4 (531-630/989aa)
• Target: ZSWIM4
• Molecular Weight: 110 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Zinc finger SWIM domain-containing protein 4; Zswim4; ZSWM4_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ZSWIM4) Polyclonal Antibody, Unconjugated (orb186509) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ZSWIM4) Polyclonal Antibody, Unconjugated (orb186509) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

ZSWIM4

UniProt

Q9H7M6

Xu Cao 1, Jinhai Tian 1, Man Yee Cheung 2, Liangying Zhang 1, Zimei Liu 3, Zongying Jiang 1 4, Shaoting Zhang 1, Kun Xiao 1, Sien Zhao 1, Ming Wang 1, Feng Ding 1, Shujing Li 1 4, Lijun Ma 5, Hui Zhao 6 7 8, Jianmin Sun Entry of ZSWIM4 to the nucleus is crucial for its inhibition of KIT and BMAL1 in gastrointestinal stromal tumors Cell Biosci ., (2024)

PubMed: 38951864

Reactivity

Mouse