ZSWIM4 Rabbit Polyclonal Antibody

• Catalog Number: orb186509
• Category: Antibodies
• Description: ZSWIM4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Porcine, Sheep
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ZSWIM4 (531-630/989aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 110 kDa
• Target: ZSWIM4
• UniProt ID: Q9H7M6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Zinc finger SWIM domain-containing protein 4; Zswi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Xu Cao 1, Jinhai Tian 1, Man Yee Cheung 2, Liangying Zhang 1, Zimei Liu 3, Zongying Jiang 1 4, Shaoting Zhang 1, Kun Xiao 1, Sien Zhao 1, Ming Wang 1, Feng Ding 1, Shujing Li 1 4, Lijun Ma 5, Hui Zhao 6 7 8, Jianmin Sun Entry of ZSWIM4 to the nucleus is crucial for its inhibition of KIT and BMAL1 in gastrointestinal stromal tumors Cell Biosci ., (2024)

PubMed: 38951864

Reactivity

Mouse

Paraformaldehyde-fixed, paraffin embedded (Rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ZSWIM4) Polyclonal Antibody, Unconjugated (orb186509) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ZSWIM4) Polyclonal Antibody, Unconjugated (orb186509) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.