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ZNF652 Antibody

SKU: orb1248806

Description

ZNF652 Antibody

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Porcine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 90kDa band observed in Human Heart, Kidney and Liver lysates (calculated MW of 69.7kDa according to NP_055712.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kumar et al, Mol Cancer Res. 2006 Sep;4(9):655-65.PMID: 1696643). Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-RHLAEKNSSAQHH
TargetZNF652
Molecular WeightApprox 90 kDa
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ZNF652, zinc finger protein 652, DKFZp781E2122, KIAA0924, OTTHUMP00000220047

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ZNF652 Antibody

orb1248806 (0.01 ug/ml) staining of Human Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

ZNF652 Antibody

orb1248806 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

ZNF652 Antibody (orb1248806)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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