ZIC2 Rabbit Polyclonal Antibody

• Catalog Number: orb575649
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ZIC2
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Predicted Reactivity: Guinea pig, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ZIC2
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 55kDa
• Target: ZIC2
• UniProt ID: O95409
• Protein Sequence: Synthetic peptide located within the following region: EPQSSSNLSPAAAAAAAAAAAAAAAVSAVHRGGGSGSGGAGGGSGGGSGS
• NCBI: NP_009060
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: HPE5
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample Tissue: Human HT1080 Whole Cell, Antibody Dilution: 1 ug/ml.

Human Skin

WB Suggested Anti-ZIC2 Antibody Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.

ZIC2 was immunoprecipitated from 1 mg HEK293 Whole Cell Lysate with orb575649 with 1:200 dilution. Western blot was performed using orb575649 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole cell lysate. Lane 2: ZIC2 IP with orb575649 in HEK293 Whole cell lysate. Lane 3: Input of HEK293 Whole cell lysate.