ZFP64 Antibody

• Catalog Number: orb2636843
• Category: Antibodies
• Description: Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZFP64 (Zinc finger protein 64), also known as ZNF338, is a 681 amino acid homolog of the mouse Zfp64 protein and is a member of the Kr ppel C2H2-type zinc-finger family. Localized to the nucleus, ZFP64 contains nine C2H2-type zinc fingers and is thought to be involved in transcriptional regulation. Four isoforms of ZFP64 exist due to alternative splicing events.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-ZFP64-1H2
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human ZFP64 protein was used as the immunogen for the ZFP64 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This ZFP64 antibody is available for research use only.
• UniProt ID: Q9NTW7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the ZFP64 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human lymph node tissue with ZFP64 antibody (clone PCRP-ZFP64-1H2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Immunofluorescent staining of PFA-fixed human HeLa cells using ZFP64 antibody (green, clone PCRP-ZFP64-1H2) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with ZFP64 antibody (blue, clone PCRP-ZFP64-1H2), and unstained cells (red).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZFP64 antibody (clone PCRP-ZFP64-1H2). These results demonstrate the foremost specificity of the PCRP-ZFP64-1H2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.