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Catalog Number | orb1151606 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to ZFP64 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | PCRP-ZFP64-1H2 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2b |
Immunogen | Recombinant full-length human ZFP64 protein was used as the immunogen for the ZFP64 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml |
Purity | Protein A/G affinity |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This ZFP64 antibody is available for research use only. |
UniProt ID | Q9NTW7 |
Storage | Aliquot the ZFP64 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the ZFP64 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human lymph node tissue with ZFP64 antibody (clone PCRP-ZFP64-1H2). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Immunofluorescent staining of PFA-fixed human HeLa cells using ZFP64 antibody (green, clone PCRP-ZFP64-1H2) and phalloidin (red).
FACS staining of PFA-fixed human HeLa cells with ZFP64 antibody (blue, clone PCRP-ZFP64-1H2), and unstained cells (red).
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using ZFP64 antibody (clone PCRP-ZFP64-1H2). These results demonstrate the foremost specificity of the PCRP-ZFP64-1H2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Human, Porcine | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |