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Catalog Number | orb1937739 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19137 |
Tested applications | FC, WB |
Predicted Reactivity | Mouse |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:2000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 136447 Da |
Target | This ZEB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1078-1105 amino acids from the C-terminal region of human ZEB2. |
UniProt ID | O60315 |
NCBI | NP_055610.1, NP_001165124.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Zinc finger E-box-binding homeobox 2, Smad-interac Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-ZEB2 Antibody (C-term) at 1:2000 dilution + Hela cell nucleus lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 136 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
ZEB2 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.