ZEB1 Mouse Monoclonal Antibody

• Catalog Number: orb783028
• Category: Antibodies
• Description: ZEB1 Mouse Monoclonal Antibody
• Target: ZEB1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1,k
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human ZEB1 (51-150/1124aa)
• UniProt ID: P37275
• MW: 124 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1:10-50
• Antibody Type: Primary Antibody
• Clone Number: Z6F8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AREB 6; AREB6; BZP; delta crystallin enhancer bind
• Note: For research use only

Blank control: A549. Primary Antibody (green line): Mouse Anti-ZEB1 antibody (orb783028), dilution: 1:50, Secondary Antibody: Goat anti-mouse IgG-PE, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Human A549 cell lysates, Primary: Anti-ZEB1 (orb783028) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 124 kD, Observed band size: 100 kD.