ZDHHC6 Rabbit Polyclonal Antibody

• Catalog Number: orb186637
• Category: Antibodies
• Description: ZDHHC6 Rabbit Polyclonal Antibody
• Target: ZDHHC6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human ZDHHC6 (221-320/413aa)
• UniProt ID: Q9H6R6
• MW: 48 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DHHC-6; FLJ21952; Probable palmitoyltransferase ZD
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (ZDHHC6) Polyclonal Antibody, Unconjugated (orb186637) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (ZDHHC6) Polyclonal Antibody, Unconjugated (orb186637) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug, Primary: Anti-ZDHHC6 (orb186637) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.

Sample: Lane 1: Mouse Pancreas tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Testis tissue lysates, Lane 4: Mouse Raw264.7 cell lysates, Lane 5: Mouse NIH/3T3 cell lysates, Lane 6: Rat Kidney tissue lysates, Lane 7: Rat Cerebrum tissue lysates, Lane 8: Rat Testis tissue lysates, Lane 9: Human HL-60 cell lysates, Primary: Anti-ZDHHC6 (orb186637) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 50 kDa.