Zcrb1 Rabbit Polyclonal Antibody

SKU: orb186701

Description

Zcrb1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Gallus, Human, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human zcrb1 (1-100/217aa)
• Target: ZCRB1
• Molecular Weight: 25 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

MADP 1; MADP1; RBM36; SNRNP31; U11/U12 small nuclear ribonucleoprotein 31 kDa protein; U11/U12 snRNP 31 kDa protein; U11/U12 snRNP 31K; U11/U12-31K; ZCCHC19; Zcrb1; ZCRB1_HUMAN; zinc finger CCHC-type and RNA binding motif 1; Zinc finger CCHC-type and RNA-binding motif-containing protein 1.

Paraformaldehyde-fixed, paraffin embedded (Mouse testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (zcrb1) Polyclonal Antibody, Unconjugated (orb186701) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Muscle (Mouse) Lysate at 40 ug, Primary: Anti-zcrb1 (orb186701) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 25 kD, Observed band size: 25 kD.

Quick Database Links

Gene Symbol

ZCRB1

UniProt

Q8TBF4