ZASP Antibody

• Catalog Number: orb1246770
• Category: Antibodies
• Description: ZASP Antibody
• Target: LDB3
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-KSQNKPEDEADE
• UniProt ID: O75112
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Western Blot experiments gave bands at approx 90kDa and 35kDa in Human Skeletal Muscle lysate after 1ug/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.1-1ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: LDB3, ZASP, LIM domain binding 3 , HGNC:15710, KIA
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_001073583.1, NP_001073585.1, NP_001073584.1, NP_009009.1

orb1246770 (0.1 ug/ml) staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1246770 (3.8 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.