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Catalog Number | orb770404 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to ZAP-70 (phospho-Tyr493) |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Immunogen | The antiserum was produced against synthesized peptide derived from human ZAP-70 around the phosphorylation site of Tyr493. AA range:460-509 |
MW | 70 |
Tested applications | ELISA, IHC-P, WB |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-ZAP70 antibody, anti-SRK antibody, anti-Tyros Read more... |
Note | For research use only |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ZAP-70 (Phospho-Tyr493) Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ZAP-70 (Phospho-Tyr493) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from Jurkat cells, using ZAP-70 (Phospho-Tyr493) Antibody. The lane on the right is blocked with the phospho peptide.
ELISA | |
Bovine, Equine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
AE |
ICC | |
Bovine, Equine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
PerCP/Cy7 |
ICC | |
Bovine, Equine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Pacific Blue |
ICC | |
Bovine, Equine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
BF700 |
ELISA, WB | |
Bovine, Equine, Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
AP |