ZAP-70 antibody

• Catalog Number: orb44588
• Category: Antibodies
• Description: Mouse Monoclonal to ZAP-70.
• Clonality: Monoclonal
• Clone Number: ZAP-03
• Tested applications: FC, ICC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP70 with histidine tag
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line. Western blotting: Recommended dilution: 0.5-2 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: ZAP70
• Entrez: 7535
• UniProt ID: P43403
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: ZAP-70
• Note: For research use only
• Application notes: Flow cytometry: Intracellular staining; recommended dilution: 2-5 μg/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line. Western blotting: Recommended dilution: 0,5 μg/ml; positive control: HPB-ALL human peripheral blood T cell leukemia cell line; negative control: RAMOS human Burkitt lymphoma cell line; reducing conditions, 10% separating gel.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 µg/ml, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 negative ZAP70 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-ZAP70 (ZAP-03) purified antibody (concentration in sample 9 µg/ml, GAM APC).

Western blotting analysis of human ZAP70 using mouse monoclonal antibody ZAP-03 on lysates of Jurkat cell line and HEK-293T cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of anti-ZAP70 mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for ZAP70 protein at approximately 70 kDa.