YWHAE Antibody

• Catalog Number: orb624375
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 14-3-3 epsilon
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:5000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:10-1:100
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• Target: YWHAE
• UniProt ID: P62258
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IP Result of anti 14-3-3 epsilon (IP: orb624375, 3 μg; Detection: orb624375 1:1000) with A375 cell lysate 5000 μg.

A375 cells were subjected to SDS PAGE followed by western blot with orb624375 (14-3-3E antibody) at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using orb624375 (14-3-3E Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).