YWHAE Rabbit Polyclonal Antibody

SKU: orb624375

Description

Rabbit polyclonal antibody to 14-3-3 epsilon

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, IP, WB
• Dilution Range: WB: 1:500-1:5000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:10-1:100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
• Target: YWHAE
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IP Result of anti 14-3-3 epsilon (IP: orb624375, 3 μg; Detection: orb624375 1:1000) with A375 cell lysate 5000 μg.

A375 cells were subjected to SDS PAGE followed by western blot with orb624375 (14-3-3E antibody) at dilution of 1:2000.

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using orb624375 (14-3-3E Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).

Quick Database Links

Gene Symbol

YWHAE

UniProt

P62258