YTHDF1 Antibody

SKU: orb1255392

Description

YTHDF1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human YTHDF1 (NP_060268.2).
• Target: YTHDF1
• Molecular Weight: Observed: 61kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C20orf21, YTH domain-containing family protein 1, DACA-1, YTH N(6)-methyladenosine RNA binding protein 1, YTH domain family 1, YTH domain family protein 1, YTH domain family, member 1, dermatomyositis associated with cancer putative autoantigen 1

Western blot analysis of extracts of various cell lines, using YTHDF1 antibody (orb1255392) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of C6 cells using YTHDF1 antibody (orb1255392) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L-929 cells using YTHDF1 antibody (orb1255392) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using YTHDF1 antibody (orb1255392) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

YTHDF1

UniProt

Q9BYJ9