XRCC5 antibody

• Catalog Number: orb19220
• Category: Antibodies
• Description: Goat polyclonal antibody to XRCC5
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 82.7
• Target: Ku80 / XRCC5
• Entrez: 7520
• Protein Sequence: SLAKKDEKTDTLED
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti XRCC5 antibody, anti KU80 antibody, anti X-ra
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration: 0.1-0.3 μg/ml. An additional band of 75kDa was observed consistent with products from other sources.
• Expiration Date: 12 months from date of receipt.

orb19220 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Spleen using XRCC5 antibody

orb19220 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.