Ku80/XRCC5 Antibody

• Catalog Number: orb259641
• Category: Antibodies
• Description: Ku80/XRCC5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Ku80 (649-676 aa FSEEQRFNNFLKALQEKVEIKQLNHFWE), different from the related mouse sequence by three amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 82705 MW
• UniProt ID: P13010
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: X-ray repair cross-complementing protein 5;3.6.4.-
• Note: For research use only
• Application notes: WB: The detection limit for Ku80 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-Ku80 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of KU80 using anti-KU80 antibody.Lane 1:Rat Testis Tissue;2:Rat Thymus Tissue;3:HELA Cell;4:NIH3T3 Cell.

IF analysis of Ku80 using anti-Ku80 antibody. Ku80 was detected in immunocytochemical section of U20S cells.

IHC analysis of KU80 using anti-KU80 antibody. KU80 was detected in a paraffin-embedded section of human lung cancer tissue.

IHC analysis of Ku80 using anti-Ku80 antibody. Ku80 was detected in frozen section of human placenta tissue.