XRCC4 antibody

• Catalog Number: orb107118
• Category: Antibodies
• Description: Rabbit polyclonal to XRCC4. DNA repair protein XRCC4 is involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. XRCC4 binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: N-terminal region of human XRCC4
• Purity: Affinity purification
• Conjugation: Unconjugated
• MW: 38
• UniProt ID: Q13426
• Storage: -20°C
• Buffer/Preservatives: 0.01% NaN3
• Alternative names: anti DNA repair protein XRCC4 antibody, anti X-ray
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot of total cell extracts from a. mouse thymus, b. human HeLa, c. human MDA-MB231; using anti-XRCC4 (N) (orb107118) at RT for 2 h.

IP-WB. Immunoprecipitation (IP) was performed with human HeLa extracts with IgG or anti-XRCC4 (N) (orb107118), followed by WB with the same Ab and Rabbit IgG light chain-specific 2nd antibody.