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Catalog Number | orb1930675 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB21824 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 28695 Da |
Target | This XBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human XBP1. |
UniProt ID | P17861 |
NCBI | NP_001073007.1, NP_005071.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | X-box-binding protein 1, XBP-1, Tax-responsive ele Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
XBP1 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
XBP1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of XBP1 Antibody (Center) in Ramos cell line lysates (35 ug/lane).XBP1 (arrow) was detected using the purified Pab.
Western blot analysis of XBP1 (arrow) using rabbit polyclonal XBP1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XBP1 gene.