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WWTR1 Rabbit Polyclonal Antibody

Catalog Number: orb574257

DispatchUsually dispatched within 3-7 working days
$ 600.00
Catalog Numberorb574257
CategoryAntibodies
DescriptionRabbit polyclonal antibody to WWTR1
TargetWWTR1
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse
Predicted ReactivityBovine, Canine, Guinea pig, Rabbit, Rat
Form/AppearanceLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration0.5 mg/ml
Buffer/PreservativesLiquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
ImmunogenThe immunogen is a synthetic peptide directed towards the N terminal region of human WWTR1
Protein SequenceSynthetic peptide located within the following region: PRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELP
UniProt IDQ9GZV5
MW44 kDa
Tested applicationsWB
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesTAZ
NoteFor research use only
NCBINP_056287
WWTR1 Rabbit Polyclonal Antibody

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Canonical 50 kDa isoform is identified.

WWTR1 Rabbit Polyclonal Antibody

Sample Tissue: Mouse Kidney, Antibody dilution: 1 ug/ml.

WWTR1 Rabbit Polyclonal Antibody

Sample Tissue: Human HepG2, Antibody dilution: 1.0 ug/ml.

WWTR1 Rabbit Polyclonal Antibody

Sample Tissue: Mouse Small Intestine, Antibody dilution: 0.5 ug/ml.

WWTR1 Rabbit Polyclonal Antibody

Sample Type: HepG2 Whole Cell lysates, Antibody dilution: 1.0 ug/ml.

WWTR1 Rabbit Polyclonal Antibody

Sample Type: Lane 1: HEK293 overexpressing WWTR1, Lane 2: Non-overexpressed vector control lysate, Lane 3: Human heart, Antibody dilution: 1.0 ug/ml.

WWTR1 Rabbit Polyclonal Antibody

Positive control (+): Mouse brain (M-BR), Negative control (-): Mouse intestine (M-IN), Antibody concentration: 1 ug/ml.

WWTR1 Rabbit Polyclonal Antibody

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WWTR1 Rabbit Polyclonal Antibody

WB Suggested Anti-WWTR1 Antibody Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.

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