WWTR1 Rabbit Polyclonal Antibody

• Catalog Number: orb574257
• Category: Antibodies
• Description: Rabbit polyclonal antibody to WWTR1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Canine, Guinea pig, Rabbit, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human WWTR1
• Concentration: 0.5 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: WWTR1
• UniProt ID: Q9GZV5
• Protein Sequence: Synthetic peptide located within the following region: PRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELP
• NCBI: NP_056287
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: TAZ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment. Canonical 50 kDa isoform is identified.

Sample Tissue: Mouse Kidney, Antibody dilution: 1 ug/ml.

Sample Tissue: Human HepG2, Antibody dilution: 1.0 ug/ml.

Sample Tissue: Mouse Small Intestine, Antibody dilution: 0.5 ug/ml.

Sample Type: HepG2 Whole Cell lysates, Antibody dilution: 1.0 ug/ml.

Sample Type: Lane 1: HEK293 overexpressing WWTR1, Lane 2: Non-overexpressed vector control lysate, Lane 3: Human heart, Antibody dilution: 1.0 ug/ml.

Positive control (+): Mouse brain (M-BR), Negative control (-): Mouse intestine (M-IN), Antibody concentration: 1 ug/ml.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/ml in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-WWTR1 Antibody Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.