WTIP Antibody

• Catalog Number: orb1269950
• Category: Antibodies
• Description: WTIP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This WTIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids of human WTIP.
• Concentration: batch dependent
• Dilution range: For IHC-P starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: WTIP
• UniProt ID: A6NIX2
• NCBI: A6NIX2
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Wilms tumor protein 1-interacting protein, WT1-int
• Note: For research use only
• Application notes: For IHC-P starting dilution is: 1:10~50For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

WTIP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in NCI-H460, WiDr cell line lysates (35 ug/lane).

Confocal immunofluorescent analysis of WTIP Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).