WNT1 Antibody

SKU: orb345596

Description

Wnt1 antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:25,000, WB: 1:1,500 - 1:6,000
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and western blotting.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Wnt1 protein.
• Target: WNT1
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-WNT-1 antibody, rabbit anti-WNT1 antibody, INT1 antibody, Murine mammary tumor virus integration site 1 antibody, Oncogene INT1 antibody, Proto oncogene protein Wnt 1 antibody, Wingless type MMTV integration site family member 1 antibody

Expression of Wnt1 in kidney homogenates of sham and nephrectomized rats. The expression of Wnt1 in kidney homogenates of sham and nephrectomized rats 18 weeks after the surgery was analyzed by immunoblotting. Blotting of GDPH served as loading control. Results are derived from 4-5 animals per group.

Western blot using Biorbyt's affinity purified anti-Wnt1 antibody shows detection of endogenous Wnt1. Lane 1: human-derived MCF7 cell lysate (p/n orb348664). Lane 2: mouse-derived 3T3 cell lysate (p/n orb348714). The band at ~41kDa, indicated by the arrowhead, corresponds to Wnt1. After transfer, the membrane was blocked with 5% BLOTTO (p/n orb348624). Primary antibody was used at a 1:1400 dilution in PBS containing 1% BLOTTO. The specificity of the antibody was confirmed by peptide competition which completely blocked reaction of the antibody with Wnt1 (data not shown).

Quick Database Links

Gene Symbol

WNT1

UniProt

P04628

RefSeq:4885655