WISP3 Rabbit Polyclonal Antibody

• Catalog Number: orb159209
• Category: Antibodies
• Description: WISP3 Rabbit Polyclonal Antibody
• Target: CCN6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Predicted Reactivity: Canine, Equine, Human, Monkey, Mouse, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human WISP3 (221-320/354aa)
• UniProt ID: O95389
• MW: 37 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CCN 6; CCN family member 6; CCN6; CYR61; LIBC; Los
• Research Area: Cell Biology, Stem Cells
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Caceres, S. et al. Canine cell line, IPC-366, as a good model for the study of inflammatory breast cancer Vet Comp Oncol, 15, 980-995 (2017)

PubMed: 27146723

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (WISP3) Polyclonal Antibody, Unconjugated (orb159209) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.