WEE1 Antibody

• Catalog Number: orb527041
• Category: Antibodies
• Description: WEE1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence of human WEE1 (ELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDR).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 2μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 72 kDa
• UniProt ID: P30291
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Wee1-like protein kinase; WEE1hu; Wee1A kinase; WE
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-WEE1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of WEE1 using anti-WEE1 antibody.Lane 1:human HeLa cell;2:human placenta tissue;3:human A431 cell;4:human U2OS cell.

IF analysis of WEE1 using anti-WEE1 antibody. WEE1 was detected in immunocytochemical section of U20S cells.

IHC analysis of WEE1 using anti-WEE1 antibody. WEE1 was detected in paraffin-embedded section of human mammary cancer tissue.