WEE1 Antibody

• Catalog Number: orb1262751
• Category: Antibodies
• Description: WEE1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This WEE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the Central region of human WEE1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 72 kDa
• Target: WEE1
• UniProt ID: P30291
• NCBI: P30291
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Wee1-like protein kinase, WEE1hu, Wee1A kinase, WE
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis in A431, Jurkat cell line lysates (35 ug/lane).This demonstrates the WEE1 antibody detected the WEE1 protein (arrow).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.