VWF Rabbit Polyclonal Antibody (PE)

SKU: orb124915

Description

VWF Rabbit Polyclonal Antibody (PE) is a PE conjugated antibody targeting VWF. This antibody is suitable for IF. It exhibits reactivity with Mouse, Rat samples.

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IF
• Dilution Range: IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human von Willebrand antigen 2 (701-800/2813aa)
• Target: VWF
• Purification: Affinity purified by Protein A
• Conjugation: PE

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Von Willebrand Factor; Coagulation factor VIII; F8VWF; Factor VIII related antigen; von Willebrand antigen 2; Von Willebrand antigen II; Von Willebrand disease; VWD; VWF_HUMAN.

Blank control: HUVEC. Primary Antibody (green line): Rabbit Anti-VWF antibody (orb124915), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

VWF