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Catalog Number | orb607810 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to B7-H4 (Immuno-Inhibitory Protein) |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | B7H4/1788 |
Tested applications | ELISA, FC, IF, IHC, WB |
Reactivity | Human |
Isotype | IgG2a |
Immunogen | A recombinant fragment of human B7-H4 protein (exact sequence is proprietary) |
Concentration | Purified Ab with BSA and Azide at 200ug/ml |
Dilution range | ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Flow Cytometry (1-2ug/million cells in 0.1ml); ,Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. |
Form/Appearance | 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Conjugation | Unconjugated |
MW | 35kDa |
Target | VTCN1 |
Entrez | 79679 |
UniProt ID | Q7Z7D3 |
Expression System | Bioreactor |
Storage | Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required. |
Buffer/Preservatives | 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml. |
Alternative names | B7 family member, H4; B7 homolog 4; B7 superfamily Read more... |
Note | For research use only |
Application notes | Positive Control: HeLa, SKBr-3 or MCF-7 cells. Human ovary, pancreas, placenta or spleen. |
Expiration Date | 12 months from date of receipt. |
FC, ICC, IF, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, WB | |
Canine, Human, Mouse, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
ELISA, FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |