VTCN1 antibody

Catalog Number: orb607810

Usually dispatched within 2 to 3 weeks.
In stock
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CategoryPrimary antibodies
Catalog Numberorb607810
Species/HostMouse
ClonalityMonoclonal
Tested applicationsELISA, FC, IF, WB, IHC
ReactivityHuman
Clone IDB7H4/1788
IsotypeIgG2a, kappa
ImmunogenA recombinant fragment of human B7-H4 protein (exact sequence is proprietary)
ConcentrationPurified Ab with BSA and Azide at 200ug/ml
Dilution rangeELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Flow Cytometry (1-2ug/million cells in 0.1ml); ,Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Form/AppearancePrepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
PurityPurified Ab with BSA and Azide at 200ug/ml
ConjugationUnconjugated
MW35 kDa
TargetVTCN1
Entrez79679
UniProt IDQ7Z7D3
StorageAntibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months
DescriptionMouse monoclonal antibody to B7-H4 (Immuno-Inhibitory Protein)
Alternative namesB7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7S1; Immune costimulatory protein B7-H4; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VCTN1(click to expand)
Expiration Date12 months from date of receipt.
NoteFor research use only.
  1. 3 images
    • Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using VTCN1 antibody.
    • Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells using VTCN1 antibody.
    • Western blot analysis of HEK293 iso 1 + iso 2 cell lysate (lane 1) + peptide (lane 2). Human Adrenal Gland iso 1 (lane 3), Human Adrenal Gland iso 2 (lane 4) and Human pancreas iso 1 (lane 5) lysate using VTCN1 antibody.
    VTCN1 antibody [orb19600]

    ELISA, FC, IF, WB

    Human

    Goat

    Polyclonal

    Unconjugated

    100 μg
  2. B7H4/VTCN1 Antibody [orb570363] Custom conjugation service available

    ELISA, FC, ICC, IF, WB, IHC

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    10 μg, 100 μg
  3. 1 image
    • Western blot analysis of Human Spleen lysate using VTCN1 antibody
    VTCN1 antibody [orb12987]

    ELISA, WB

    Human

    Goat

    Polyclonal

    Unconjugated

    100 μg
  4. 1 image
    • Western blot analysis of extracts of various cell lines lysates using VTCN1 antibody
    VTCN1 antibody [orb542329]

    WB

    Human, Mouse, Rat

    Polyclonal

    50 μl, 100 μl, 200 μl
  5. 1 image
    VTCN1 antibody [orb40506]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    400 μl, 80 μl