VTCN1 antibody

• Catalog Number: orb19600
• Category: Antibodies
• Description: Goat polyclonal antibody to VTCN1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 30.9; 20.7
• Target: B7-H4
• Entrez: 79679
• Protein Sequence: SKGKGNANLEYK
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti B7-H4 antibody, anti B7H4 antibody, anti B7S1
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 60kDa band observed in Human Lung, Spleen and Thymus lysates (calculated MW of 30.9kDa according to NP_078902.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Simon et al, Cancer Res. 2006 Feb 1;66(3):1570-5.; PMID: 16452214). In transfected HEK293 transiently expressing B7-H4 the band of approx. 60kDa is also observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells using VTCN1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells using VTCN1 antibody.

Western blot analysis of HEK293 iso 1 + iso 2 cell lysate (lane 1) + peptide (lane 2). Human Adrenal Gland iso 1 (lane 3), Human Adrenal Gland iso 2 (lane 4) and Human pancreas iso 1 (lane 5) lysate using VTCN1 antibody.