Vsir Antibody

• Catalog Number: orb1671801
• Category: Antibodies
• Description: Vsir Antibody
• Clonality: Recombinant
• Clone Number: MH5A
• Tested applications: Blocking, ELISA, IHC
• Reactivity: Mouse
• Isotype: IgG lambda
• Immunogen: The antibody was generated by immunising Armenian hamsters with mPD-1HIg.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Vsir
• UniProt ID: Q9D659
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: VISTA, VISR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA of anti-VISTA antibody on VISTA-Fc fusion protein. Binding curve of the rmeric version of the anti-VISTA antibody MH5A (orb1671801; blue line) to an ELISA plate coated with mouse VISTA-Fc fusion protein (Pr00164-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 1.7 ng/ml was performed using orb1671801. For signal detection- a 1:4000 dilution of anti-r1 HRP antibody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-PD-1H antibody MH5A. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes permewith 0.15% Triton and stained with the chimeric mouse IgG1 version of MH5A (orb1671801) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671801- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody (0) followed by Alexa Fluor® 488 secondary antibody.