VMP1 Antibody (RPE)

SKU: orb384230

Description

Rabbit polyclonal antibody against VMP1 conjugated to RPE.

Images & Validation

• Tested Applications: ICC, IF, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human
• Application Notes:
  A 1:1000 dilution of SPC-680 was sufficient for detection of VMP1 in 15 µg of Human 293T Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-terminal of Human VMP1 (aa. 391-402)
• Target: VMP1
• Molecular Weight: 46 kDa
• Purification: Peptide Affinity Purified
• Conjugation: RPE

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

VMP1, Vacuole Membrane Protein 1, TMEM49, Transmembrane Protein 49, TANGO5, Transport And Golgi Organization 5 Homolog, EPG3, Ectopic P-Granules Autophagy Protein 3 Homolog, TDC1, HSPC292

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-VMP1 Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane, Vacuole Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VMP1 Antibody. (D) Composite.

Western blot analysis of Human A549 showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: A549 cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa.

Quick Database Links

Gene Symbol

VMP1

UniProt

Q96GC9

NCBI Gene ID

81671

RefSeq (Protein):NP_112200.2