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VIPR2 Rabbit Polyclonal Antibody

Catalog Number: orb11218

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11218
CategoryAntibodies
DescriptionVIPR2 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityMouse, Rabbit, Rat
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from rat VIP receptor II (81-170/437aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW48 kDa
TargetVIPR2
RRIDAB_10751844
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesVIP receptor II; VIP receptor2; VIP receptor-2; PA
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NoteFor research use only
Expiration Date12 months from date of receipt.
VIPR2 Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-VPAC2R antibody (orb11218), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 2 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

VIPR2 Rabbit Polyclonal Antibody

Sample: A431 Cell Lysate at 40 ug, Primary: Anti-VPAC2R (orb11218) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 63 kD.

VIPR2 Rabbit Polyclonal Antibody

Sample: Lane 1: Recombinant human VIPR2 protein, N-Trx-His, Primary: Anti-VIPR2 (orb11218) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kDa, Observed band size: 34 kDa.

VIPR2 Rabbit Polyclonal Antibody

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-VIP receptor 2/VPAC2 Polyclonal Antibody, Unconjugated (orb11218) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • VIPR2 Rabbit Polyclonal Antibody (PE) [orb495275]

    FC,  IF

    Mouse, Rabbit, Rat

    Human

    Rabbit

    Polyclonal

    PE

    100 μl
  • VPAC2 rabbit pAb [orb770385]

    ELISA,  IF,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    50 μl, 100 μl
  • VIPR2 Antibody (N-term) [orb1934422]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • VIPR2 Rabbit Polyclonal Antibody [orb2563151]

    IF,  IHC-Fr,  IHC-P

    Human

    Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 200 μl
  • VIPR2 Rabbit Polyclonal Antibody (HRP) [orb479408]

    IHC-Fr,  IHC-P,  WB

    Mouse, Rabbit, Rat

    Human

    Rabbit

    Polyclonal

    HRP

    100 μl