Vimentin Rabbit Polyclonal Antibody (FITC)

SKU: orb16490

Description

Vimentin Rabbit Polyclonal Antibody (FITC)

Images & Validation

• Tested Applications: FC, ICC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:100-500, ICC/IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Goat, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Vimentin (371-466/466aa)
• Target: VIM
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

FLJ36605; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.

Blank control: U2OS. Primary Antibody (green line): Rabbit Anti-VIM antibody (orb16490), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

VIM