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Catalog Number | orb1258103 |
---|---|
Category | Antibodies |
Description | VIM Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin (NP_003371.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 55kDa |
Target | VIM |
UniProt ID | P08670 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | Vimentin, VIM Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using Vimentin antibody (orb1258103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using Vimentin antibody (orb1258103) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Vimentin antibody (orb1258103) at dilution of 1:100. Blue: DAPI for nuclear staining.
IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, WB | |
Bovine, Canine, Equine, Porcine, Rat |
ELISA, FC, IF, IHC, WB | |
Bovine, Canine, Human, Mouse, Porcine, Rat | |
Goat | |
Polyclonal | |
Unconjugated |
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