VIL1 Antibody

• Catalog Number: orb1252229
• Category: Antibodies
• Description: VIL1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: VIL1/1325
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlWestern blot: 1-2 ug/mlIHC (FFPE): 0.25-0.5 ug/ml for 30 min at RTThe stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: VIL1
• UniProt ID: P09327
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: VIL1, D2S1471, Villin, Villin 1, Villin-1, VIL
• Note: For research use only
• Application notes: Flow Cytometry: 0.5-1ug/10^6 cells in 0.1mlIF: 1-2 ug/mlWestern blot: 1-2 ug/mlIHC (FFPE): 0.25-0.5 ug/ml for 30 min at RTThe stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human colon with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1325). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human 1) A549, 2) HepG2 and 3) HCT-116 cell lysate with Villin antibody (clone VIL1/1325). Expected molecular weight ~93 kDa.

SDS-PAGE Analysis of Purified, BSA-Free Villin Antibody (clone VIL1/1325). Confirmation of Integrity and Purity of the Antibody.